[Cloning of ACA gene promoter and preliminary study of its function].
Identifieur interne : 000776 ( Main/Exploration ); précédent : 000775; suivant : 000777[Cloning of ACA gene promoter and preliminary study of its function].
Auteurs : Zhao-Hua Liu [République populaire de Chine] ; Hong-Nian Guo ; Guang-Yu Zheng ; Ying-Chuan TianSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2005.
Descripteurs français
- KwdFr :
- Amaranthus (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Escherichia coli (métabolisme), Lectines végétales (génétique), Rhizobium (génétique), Rhizobium (métabolisme), Régions promotrices (génétique) (génétique), Séquence nucléotidique (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Lectins.
- genetics : Amaranthus, Escherichia coli, Promoter Regions, Genetic, Rhizobium.
- metabolism : Escherichia coli, Rhizobium.
- Base Sequence, Cloning, Molecular, Molecular Sequence Data.
Abstract
Using total DNA isolated from Amaranthus caudatus as the template, a DNA fragment of about 700bp upstream of the coding sequence of Amaranthus caudatus agglutinin (ACA) gene was amplified by TAIL-PCR and cloned. To examine the regulatory function of this DNA fragment, it was inserted into a plant expression vector containing GUS gene to substitute the CaMV 35S promoter and the resulted recombinant plasmid was designated as pBpAG. The expression vector pBpAG was transferred to different tissues of plants, via Agrobacterium-mediated transformation in vacuum condition. Transient expression of GUS in the transformed tissues was detected by histochemical GUS staining and the results showed that the GUS activity was expressed specifically in seeds. These preliminary results indicate that this DNA fragment upstream of the ACA coding sequence could very possibly be a promoter with seed specificity. Some putative cis-elements within the promoter were discussed.
PubMed: 15859344
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Zheng">Guang-Yu Zheng</name></author><author><name sortKey="Tian, Ying Chuan" sort="Tian, Ying Chuan" uniqKey="Tian Y" first="Ying-Chuan" last="Tian">Ying-Chuan Tian</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15859344</idno><idno type="pmid">15859344</idno><idno type="wicri:Area/Main/Corpus">000792</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000792</idno><idno type="wicri:Area/Main/Curation">000792</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000792</idno><idno type="wicri:Area/Main/Exploration">000792</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Cloning of ACA gene promoter and preliminary study of its function].</title><author><name sortKey="Liu, Zhao Hua" sort="Liu, Zhao Hua" uniqKey="Liu Z" first="Zhao-Hua" last="Liu">Zhao-Hua Liu</name><affiliation wicri:level="1"><nlm:affiliation>Institution of Microbiology Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institution of Microbiology Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Guo, Hong Nian" sort="Guo, Hong Nian" uniqKey="Guo H" first="Hong-Nian" last="Guo">Hong-Nian Guo</name></author><author><name sortKey="Zheng, Guang Yu" sort="Zheng, Guang Yu" uniqKey="Zheng G" first="Guang-Yu" last="Zheng">Guang-Yu Zheng</name></author><author><name sortKey="Tian, Ying Chuan" sort="Tian, Ying Chuan" uniqKey="Tian Y" first="Ying-Chuan" last="Tian">Ying-Chuan Tian</name></author></analytic><series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title><idno type="ISSN">1000-3061</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amaranthus (genetics)</term><term>Base Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Lectins (genetics)</term><term>Promoter Regions, Genetic (genetics)</term><term>Rhizobium (genetics)</term><term>Rhizobium (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amaranthus (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Lectines végétales (génétique)</term><term>Rhizobium (génétique)</term><term>Rhizobium (métabolisme)</term><term>Régions promotrices (génétique) (génétique)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Lectins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amaranthus</term><term>Escherichia coli</term><term>Promoter Regions, Genetic</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amaranthus</term><term>Escherichia coli</term><term>Lectines végétales</term><term>Rhizobium</term><term>Régions promotrices (génétique)</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Rhizobium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cloning, Molecular</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using total DNA isolated from Amaranthus caudatus as the template, a DNA fragment of about 700bp upstream of the coding sequence of Amaranthus caudatus agglutinin (ACA) gene was amplified by TAIL-PCR and cloned. To examine the regulatory function of this DNA fragment, it was inserted into a plant expression vector containing GUS gene to substitute the CaMV 35S promoter and the resulted recombinant plasmid was designated as pBpAG. The expression vector pBpAG was transferred to different tissues of plants, via Agrobacterium-mediated transformation in vacuum condition. Transient expression of GUS in the transformed tissues was detected by histochemical GUS staining and the results showed that the GUS activity was expressed specifically in seeds. These preliminary results indicate that this DNA fragment upstream of the ACA coding sequence could very possibly be a promoter with seed specificity. Some putative cis-elements within the promoter were discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15859344</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>22</Day></DateCompleted><DateRevised><Year>2005</Year><Month>04</Month><Day>29</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-3061</ISSN><JournalIssue CitedMedium="Print"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Sheng wu gong cheng xue bao = Chinese journal of biotechnology</Title><ISOAbbreviation>Sheng Wu Gong Cheng Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Cloning of ACA gene promoter and preliminary study of its function].</ArticleTitle><Pagination><MedlinePgn>139-43</MedlinePgn></Pagination><Abstract><AbstractText>Using total DNA isolated from Amaranthus caudatus as the template, a DNA fragment of about 700bp upstream of the coding sequence of Amaranthus caudatus agglutinin (ACA) gene was amplified by TAIL-PCR and cloned. To examine the regulatory function of this DNA fragment, it was inserted into a plant expression vector containing GUS gene to substitute the CaMV 35S promoter and the resulted recombinant plasmid was designated as pBpAG. The expression vector pBpAG was transferred to different tissues of plants, via Agrobacterium-mediated transformation in vacuum condition. Transient expression of GUS in the transformed tissues was detected by histochemical GUS staining and the results showed that the GUS activity was expressed specifically in seeds. These preliminary results indicate that this DNA fragment upstream of the ACA coding sequence could very possibly be a promoter with seed specificity. Some putative cis-elements within the promoter were discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Zhao-Hua</ForeName><Initials>ZH</Initials><AffiliationInfo><Affiliation>Institution of Microbiology Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Hong-Nian</ForeName><Initials>HN</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Guang-Yu</ForeName><Initials>GY</Initials></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Ying-Chuan</ForeName><Initials>YC</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Sheng Wu Gong Cheng Xue Bao</MedlineTA><NlmUniqueID>9426463</NlmUniqueID><ISSNLinking>1000-3061</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037121">Plant Lectins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D027721" MajorTopicYN="N">Amaranthus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037121" MajorTopicYN="N">Plant Lectins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>4</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>7</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>4</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15859344</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Guo, Hong Nian" sort="Guo, Hong Nian" uniqKey="Guo H" first="Hong-Nian" last="Guo">Hong-Nian Guo</name><name sortKey="Tian, Ying Chuan" sort="Tian, Ying Chuan" uniqKey="Tian Y" first="Ying-Chuan" last="Tian">Ying-Chuan Tian</name><name sortKey="Zheng, Guang Yu" sort="Zheng, Guang Yu" uniqKey="Zheng G" first="Guang-Yu" last="Zheng">Guang-Yu Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Zhao Hua" sort="Liu, Zhao Hua" uniqKey="Liu Z" first="Zhao-Hua" last="Liu">Zhao-Hua Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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